antibodies against drd1  (Bioss)

Journal: Frontiers in Pharmacology

Article Title: Cannabidiol prevents methamphetamine-induced neurotoxicity by modulating dopamine receptor D1-mediated calcium-dependent phosphorylation of methyl-CpG-binding protein 2

doi: 10.3389/fphar.2022.972828

Figure Lengend Snippet: Protein-drug binding simulations. Seven transmembrane domains of DRD1 are shown as multicolor bands. METH or CBD are marked in cyan, while key DRD1 residues are marked in yellow. (A) Docking pose, binding sites, and interactions between DRD1 model and METH. (B) Docking pose, binding sites, and interactions between DRD1 model and CBD. (C) Docking pose, binding sites, and interactions among CBD, METH, and DRD1 model.

Article Snippet: The neurons were then incubated with PBS containing antibodies against DRD1 (Bio-Techne, #NB110-60017, 1:200, Minneapolis, MN, United States), pMeCP2 (phospho Ser421) (Abcam, #ab254050, 1:200, Cambridge, United Kingdom), cleaved caspase-8 (Proteintech, #66093-1-Ig, 1:200, IL, United States), or cleaved caspase-3 (Cell Signaling Technology, #9664, 1:200, MA, United States) overnight at 4°C.

Techniques: Binding Assay

Journal: Frontiers in Pharmacology

Article Title: Cannabidiol prevents methamphetamine-induced neurotoxicity by modulating dopamine receptor D1-mediated calcium-dependent phosphorylation of methyl-CpG-binding protein 2

doi: 10.3389/fphar.2022.972828

Figure Lengend Snippet: Protein levels and colocalization in neurons incubated with CBD and/or METH. (A) Levels of pMeCP2 and apoptosis-related proteins. (B) DRD1 and pMeCP2 levels and their colocalization. (C) Cleaved caspase-8 and cleaved caspase-3 levels and their colocalization. ** p < 0.01 or *** p < 0.001 compared with control group, # p < 0.05 or ### p < 0.001 compared with METH group; Tukey HSD post-hoc comparison after significant ANOVA.

Article Snippet: The neurons were then incubated with PBS containing antibodies against DRD1 (Bio-Techne, #NB110-60017, 1:200, Minneapolis, MN, United States), pMeCP2 (phospho Ser421) (Abcam, #ab254050, 1:200, Cambridge, United Kingdom), cleaved caspase-8 (Proteintech, #66093-1-Ig, 1:200, IL, United States), or cleaved caspase-3 (Cell Signaling Technology, #9664, 1:200, MA, United States) overnight at 4°C.

Techniques: Incubation, Comparison

Journal: Frontiers in Pharmacology

Article Title: Cannabidiol prevents methamphetamine-induced neurotoxicity by modulating dopamine receptor D1-mediated calcium-dependent phosphorylation of methyl-CpG-binding protein 2

doi: 10.3389/fphar.2022.972828

Figure Lengend Snippet: Protein levels and colocalization in neurons incubated with SCH23390 and/or METH. (A) Levels of pMeCP2 and apoptosis-related proteins. (B) DRD1 and pMeCP2 levels and their colocalization. (C) Cleaved caspase-8 and cleaved caspase-3 levels and their colocalization. ** p < 0.01 or *** p < 0.001 compared with control group, ## p < 0.01 or ### p < 0.001 compared with METH group, && p < 0.01 or &&& p < 0.001 compared with SCH23390 group; Tukey HSD post-hoc comparison after significant ANOVA.

Article Snippet: The neurons were then incubated with PBS containing antibodies against DRD1 (Bio-Techne, #NB110-60017, 1:200, Minneapolis, MN, United States), pMeCP2 (phospho Ser421) (Abcam, #ab254050, 1:200, Cambridge, United Kingdom), cleaved caspase-8 (Proteintech, #66093-1-Ig, 1:200, IL, United States), or cleaved caspase-3 (Cell Signaling Technology, #9664, 1:200, MA, United States) overnight at 4°C.

Techniques: Incubation, Comparison

Journal: Frontiers in Pharmacology

Article Title: Cannabidiol prevents methamphetamine-induced neurotoxicity by modulating dopamine receptor D1-mediated calcium-dependent phosphorylation of methyl-CpG-binding protein 2

doi: 10.3389/fphar.2022.972828

Figure Lengend Snippet: Protein levels and colocalization in neurons incubated with CBD and/or SKF81297. (A) Levels of pMeCP2 and apoptosis-related proteins. (B) DRD1 and pMeCP2 levels and their colocalization. (C) Cleaved caspase-8 and cleaved caspase-3 levels and their colocalization. ** p < 0.01 or *** p < 0.001 compared with control group, ## p < 0.01 or ### p < 0.001 compared with METH group; Tukey HSD post-hoc comparison after significant ANOVA.

Article Snippet: The neurons were then incubated with PBS containing antibodies against DRD1 (Bio-Techne, #NB110-60017, 1:200, Minneapolis, MN, United States), pMeCP2 (phospho Ser421) (Abcam, #ab254050, 1:200, Cambridge, United Kingdom), cleaved caspase-8 (Proteintech, #66093-1-Ig, 1:200, IL, United States), or cleaved caspase-3 (Cell Signaling Technology, #9664, 1:200, MA, United States) overnight at 4°C.

Techniques: Incubation, Comparison

Journal: Frontiers in Pharmacology

Article Title: Cannabidiol prevents methamphetamine-induced neurotoxicity by modulating dopamine receptor D1-mediated calcium-dependent phosphorylation of methyl-CpG-binding protein 2

doi: 10.3389/fphar.2022.972828

Figure Lengend Snippet: Protein, intracellular Ca 2+ , and apoptosis levels in prefrontal cortex of rats repeatedly exposed to CBD and/or METH. (A) Levels of DRD1, pMeCP2, and apoptosis-related proteins in prefrontal cortex. (B) Intracellular Ca 2+ level in prefrontal cortex. (C) Apoptosis level in prefrontal cortex. *** p < 0.001 compared with control group, ### p < 0.001 compared with METH group; Tukey HSD post-hoc comparison after significant ANOVA.

Article Snippet: The neurons were then incubated with PBS containing antibodies against DRD1 (Bio-Techne, #NB110-60017, 1:200, Minneapolis, MN, United States), pMeCP2 (phospho Ser421) (Abcam, #ab254050, 1:200, Cambridge, United Kingdom), cleaved caspase-8 (Proteintech, #66093-1-Ig, 1:200, IL, United States), or cleaved caspase-3 (Cell Signaling Technology, #9664, 1:200, MA, United States) overnight at 4°C.

Techniques: Comparison

Journal: Frontiers in Pharmacology

Article Title: Cannabidiol prevents methamphetamine-induced neurotoxicity by modulating dopamine receptor D1-mediated calcium-dependent phosphorylation of methyl-CpG-binding protein 2

doi: 10.3389/fphar.2022.972828

Figure Lengend Snippet: Protein, intracellular Ca 2+ , and apoptosis levels in hippocampus of rats repeatedly exposed to CBD and/or METH. (A) Levels of DRD1, pMeCP2, and apoptosis-related proteins in hippocampus. (B) Intracellular Ca 2+ level in hippocampus. (C) Apoptosis level in hippocampus. *** p < 0.001 compared with control group, ### p < 0.001 compared with METH group; Tukey HSD post-hoc comparison after significant ANOVA.

Article Snippet: The neurons were then incubated with PBS containing antibodies against DRD1 (Bio-Techne, #NB110-60017, 1:200, Minneapolis, MN, United States), pMeCP2 (phospho Ser421) (Abcam, #ab254050, 1:200, Cambridge, United Kingdom), cleaved caspase-8 (Proteintech, #66093-1-Ig, 1:200, IL, United States), or cleaved caspase-3 (Cell Signaling Technology, #9664, 1:200, MA, United States) overnight at 4°C.

Techniques: Comparison

Journal: Acta Pharmacologica Sinica

Article Title: Isosibiricin inhibits microglial activation by targeting the dopamine D1/D2 receptor-dependent NLRP3/caspase-1 inflammasome pathway

doi: 10.1038/s41401-019-0296-7

Figure Lengend Snippet: Isosibiricin upregulates dopamine D1/2 receptors expressions in LPS-induced BV-2 cells. a , b The predicted pathways ( a ) and targets ( b ) of isosibiricin inferred by the differential expressed gene-based method. c , d Isosibiricin upregulated dopamine D1 receptors ( c ) and dopamine D2 receptor ( d ) expressions in LPS-induced BV-2 cells. ## P < 0.01 vs. control group. ** P < 0.01 vs. LPS group

Article Snippet: Antibodies against DRD1 and DRD2 were obtained from Bioss (Beijing, China).

Techniques:

Journal: Free radical biology & medicine

Article Title: Dopamine D1 receptor agonist alleviates acute lung injury via modulating inflammatory responses in macrophages and barrier function in airway epithelial cells.

doi: 10.1016/j.freeradbiomed.2023.03.016

Figure Lengend Snippet: Fig. 1. Decreased dopamine D1-like receptors expression in macrophages and airway epithelial cells of LPS-induced ALI mouse model. (A, B) LPS challenge (10 mg/kg) dramatically elevated the counts of total inflammatory cells, neutrophils, macrophages, and lymphocytes (A) as well as levels of cytokines IL-6, KC, G-CSF and TNF-α (B) in the BALF of ALI mice. (C) Histopathology images indicating obvious lung damage in LPS-treated mice. Scale bar: 40 μm. (D–H) Immunohisto chemical assay and the quantitative analysis (H) showing the decreased expression of dopamine D1-like receptors DRD1 (D, E) and DRD5 (F, G) in pulmonary macrophages (red arrow) and bronchial epithelial cells (BECs). Scale bar: 40 μm. n = 6. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns: not significant. Error bars represented mean ± SEM. Statistical analysis was performed using two-tailed unpaired Student t-test. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: For the IHC method, lung tissue slides were stained with the primary antibodies against DRD1 (Proteintech, China) and DRD5 (Bioss, China) and then incubated with goat anti-rabbit IgG secondary antibody.

Techniques: Expressing, Histopathology, Two Tailed Test

Journal: Frontiers in Neuroscience

Article Title: Regional Downregulation of Dopamine Receptor D1 in Bilateral Dorsal Lateral Geniculate Nucleus of Monocular Form-Deprived Amblyopia Models

doi: 10.3389/fnins.2022.861529

Figure Lengend Snippet: List of primers for qPCR of monkey samples.

Article Snippet: Then, the sections were incubated in the following order: Primary antibodies against DRD1 (GeneTex, GTX100355) overnight at 4°C, Alexa Fluor 488-conjugated secondary antibody for 1 h at RT, and 0.1% DAPI for 5 min at RT, with washing in PBS 3 times after each incubation.

Techniques:

Journal: Frontiers in Neuroscience

Article Title: Regional Downregulation of Dopamine Receptor D1 in Bilateral Dorsal Lateral Geniculate Nucleus of Monocular Form-Deprived Amblyopia Models

doi: 10.3389/fnins.2022.861529

Figure Lengend Snippet: List of primers for qPCR of mouse samples.

Article Snippet: Then, the sections were incubated in the following order: Primary antibodies against DRD1 (GeneTex, GTX100355) overnight at 4°C, Alexa Fluor 488-conjugated secondary antibody for 1 h at RT, and 0.1% DAPI for 5 min at RT, with washing in PBS 3 times after each incubation.

Techniques:

Journal: Frontiers in Neuroscience

Article Title: Regional Downregulation of Dopamine Receptor D1 in Bilateral Dorsal Lateral Geniculate Nucleus of Monocular Form-Deprived Amblyopia Models

doi: 10.3389/fnins.2022.861529

Figure Lengend Snippet: Receptors selected for verification that were associated with neural development.

Article Snippet: Then, the sections were incubated in the following order: Primary antibodies against DRD1 (GeneTex, GTX100355) overnight at 4°C, Alexa Fluor 488-conjugated secondary antibody for 1 h at RT, and 0.1% DAPI for 5 min at RT, with washing in PBS 3 times after each incubation.

Techniques:

Journal: Frontiers in Neuroscience

Article Title: Regional Downregulation of Dopamine Receptor D1 in Bilateral Dorsal Lateral Geniculate Nucleus of Monocular Form-Deprived Amblyopia Models

doi: 10.3389/fnins.2022.861529

Figure Lengend Snippet: DRD1 is downregulated in the LGN of monocularly deprived amblyopic mice. (A) Western blot of samples from mouse LGN. (B) Statistics of the relative expression level of DRD1. Set the value of intact LGN as 1. Data represent the mean ± SE. n = 3, * p < 0.05.

Article Snippet: Then, the sections were incubated in the following order: Primary antibodies against DRD1 (GeneTex, GTX100355) overnight at 4°C, Alexa Fluor 488-conjugated secondary antibody for 1 h at RT, and 0.1% DAPI for 5 min at RT, with washing in PBS 3 times after each incubation.

Techniques: Western Blot, Expressing

Journal: Frontiers in Neuroscience

Article Title: Regional Downregulation of Dopamine Receptor D1 in Bilateral Dorsal Lateral Geniculate Nucleus of Monocular Form-Deprived Amblyopia Models

doi: 10.3389/fnins.2022.861529

Figure Lengend Snippet: Downregulation of DRD1 in dLGNs in the target regions of mouse amblyopic eyes. (A1–A4) Anti-DRD1 immunostaining in the dLGN. The dashed white line delineates the dLGN. The shell and core regions are separated with a dashed red line in (A1) . Six ROIs (region of interest) in (A2) represent the areas for measuring and statistics. (B1–B4) Grayscale image of anti-DRD1 immunostaining for statistics. The most downregulated areas are indicated with a dashed blue circle in (B3) and a dashed red circle in (B4) . (C) Quantification of the normalized fluorescence intensity of DRD1 staining. Data represent the mean ± SE, n = 8 for the intact group, n = 6 for both ipsilateral and contralateral groups, * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Then, the sections were incubated in the following order: Primary antibodies against DRD1 (GeneTex, GTX100355) overnight at 4°C, Alexa Fluor 488-conjugated secondary antibody for 1 h at RT, and 0.1% DAPI for 5 min at RT, with washing in PBS 3 times after each incubation.

Techniques: Immunostaining, Fluorescence, Staining

Journal: Frontiers in Neuroscience

Article Title: Electroacupuncture Attenuates Neuropathic Pain and Comorbid Negative Behavior: The Involvement of the Dopamine System in the Amygdala

doi: 10.3389/fnins.2021.657507

Figure Lengend Snippet: The effects of EA on DRD1 and DRD2 protein expression in the amygdala. (A) Western blot gel images showing protein levels of DRD1 and DRD2 in the amygdala. Quantification of DRD1 (B) and DRD2 (C) in the amygdala, respectively ( n = 3 per group). Representative immunofluorescence staining images of DRD1 (D) and DRD2 (E) in the amygdala sections and signal intensity quantitation (F) are shown (scale bar = 50 μm) ( n = 4 per group). Data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. the sham group; # p < 0.05, ## p < 0.01 vs. the CCI group.

Article Snippet: For immunofluorescence, the sections were incubated with a primary antibody directed against DRD1 (1:1,000) or DRD2 (1:300) overnight at 4°C, followed by an incubation with fluorescent-labeled goat anti-rabbit or goat anti-mouse IgG (1:600, Servicebio Technology Co., Ltd.).

Techniques: Expressing, Western Blot, Immunofluorescence, Staining, Quantitation Assay